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Membranes, detergents, and receptor solubilization

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Published by A.R. Liss in New York .
Written in English

Subjects:

  • Cell membranes.,
  • Cell receptors.,
  • Solubilization.,
  • Surface active agents.

Book details:

Edition Notes

Includes bibliographies and index.

Statementeditors, J. Craig Venter, Len C. Harrison.
SeriesReceptor biochemistry and methodology ;, v. 1
ContributionsVenter, J. Craig., Harrison, Len C.
Classifications
LC ClassificationsQH601 .M4819 1984
The Physical Object
Paginationxii, 225 p. :
Number of Pages225
ID Numbers
Open LibraryOL3173962M
ISBN 10084513700X
LC Control Number83016271

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Membranes, Detergents, and Receptor Solubilization by J. Craig Venter, Len C. Harrison starting at $ Membranes, Detergents, and Receptor Solubilization has 1 available editions to buy at Half Price Books Marketplace. PDF | On , Tessie McNeely and others published Receptor Biochemistry and Methodology. Volume 1: Membranes, Detergents, and Receptor Solubilization. Volume 2: Receptor Purification. solubilization of membrane proteins are highlighted with the solubilization of the serotonin 1A receptor taken as a specific example. IUBMB Life, –, Keywords CHAPS; detergent; membrane lipid environment; mem-brane protein; serotonin 1A receptor; solubilization. INTRODUCTION Biological membranes are complex assemblies of a diverseCited by:   Schematic representation of the novel systematic membrane proteins solubilization method (BMSS for Biotinylated Membranes Solubilization and Separation). 4 steps are described. 1-Insertion of biotinylated lipids into biological membranes. 2- Isolation of biotinylated biological membranes using streptavidine magnetic beads after sucrose gradient. 3- Membrane protein solubilization using detergents above the critical Micelle Concentration (10CMC). 4- Solubilized membrane .

tion, membrane dissolution, inclusion body solubilization, lipid raft preparation, and membrane protein solubilization, biochemistry, crystallization, and manipulation. Detergents are also useful as model membranes for in vitro studies and as vehicles for protein/DNA/drug delivery. detergents for membrane protein applications, understanding the solubilization, lipid raft preparation, and membrane protein solubi-lization, biochemistry, crystallization, and manipulation. Detergents receptor, a member of the GPCR superfamily, Berger et al. showed that detergents with an. Bruns RF, Lawson-Wendling K, Pugsley TA. A rapid filtration assay for soluble receptors using polyethylenimine-treated filters. Anal Biochem. Jul 1; (1)– Cuatrecasas P. Isolation of the insulin receptor of liver and fat-cell membranes (detergent-solubilized-( I)insulin-polyethylene glycol precipitation-sephadex). 10 detergents used for the structural analysis of membrane proteins based on the published results. The aim of this study is to provide the reader with an overview of the main properties of available detergents (critical micelle concentration (CMC) value, micelle size, etc.) and provide an idea of what.

Staudinger R., Bandrés J.C. () Solubilization of Chemokine Receptors from Cell Membranes. In: Selinsky B.S. (eds) Membrane Protein Protocols. Methods in Molecular Biology, vol Humana Press. DOI ; Publisher Name Humana Press; Print ISBN ; Online ISBN Detergents are widely used in biochemistry, cell biology and molecular biology. Common applications include cell lysis, solubilization of membrane proteins and lipids, protein crystallization, and reduction of background staining in blotting experiments. Figure 1: A phospholipid bilayer. Even though studying membrane proteins is a major challenge in protein biochemistry, they remain an important area of . detergents. However, solubilization of opioid receptors has been rather more difficult, owing to their extreme sensitivity to detergents. Brij 36T was used to solubilize eH] etorphine labelled opioid receptor complex from rat brain # Author for correspondence synaptosomal fraction3. Extraction of the membrane bound opioid receptors into solution was tried by sonication4. Receptor Biochemistry and Methodology. Volume 1: Membranes, Detergents, and Receptor Solubilization. Volume 2: Receptor Purification Procedures. Volume 3: Molecular and Chemical Characterization of Membrane Receptors.